AF488 azide | 1679326-36-3 (with azidohexyl group)

Product Introduction

AF488 is a fluorescent dye. AF488 is a fluorophore pH-insensitive over a broad pH range (from 4 to 10). It has an absorption maximum at 495 nm and an emission maximum at 519 nm in the green spectrum region. The dye is hydrophilic and can be used to introduce the fluorescent label into various molecules, including proteins and antibodies. Conjugates of molecules with AF488 have high brightness and photostability and are commonly used in flow cytometry and microscopy. This allows the detection of biological objects with high sensitivity at a longer imaging time.,AF488 azide interacts with alkynyl derivatives of biomolecules in Click Chemistry reactions either in the presence of copper (I) catalyst (with terminal alkynes) or without catalyst (with cyclooctynes), leading to the formation of stable adducts.

Chemical Information
Purity	NMR 1H, HPLC-MS (95%)
Solubility	Good in water, DMF, DMSO
Appearance	Orange solid

Product Specification
CF260	0.16
CF280	0.10
Fluorescence Quantum Yield	0.91
Mass Spec M+ Increment	616.1
Excitation	495
Emission	519
Storage	Storage: 24 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.